![]() ![]() I also noticed that the file path for hashing is messed up. Since I just installed 490, I presume that the CheckRevision file is as updated as possible. Hashing is also failing for some reason, claiming that in cannot read something from CheckRevision.ini. I was previously on build 482, bu have updated to 490 and the problem still persists. If I leave all the settings the same ( & ) and switch to a D2 key, the bot will connect just fine. I've tried switching IP addresses and BNLS servers but it keeps hanging. It has started hanging on Checking Version. If the mixture is very polar, the pre-adsorbtion procedure is preferable as the use of too much EtOAc will compromise the resolution.This issue started around 24 hours ago, or that was the last time I was able to connect with W2BN. If necessary a small amount of EtOAc can be added to dissolve more polar compounds. 11įor small columns (< 4 cm) it is usually easier to dissolve the mixture in a small volume of n-heptane and add it evenly onto the surface of the column with a pipette. ![]() 40 g on a column with a diameter of 10 cm) and still achieve good separation of mixtures with a ΔR f ≅ 0.05 (by analytical TLC). In general we have found that it is possible to load a mixture of up to approx. All chromatography should be performed in a hood behind a safety shield. Special care should be taken when using a separatory funnel as it is not designed for this purpose. The inventors of Flash Column Chromatography stressed on the fact that Flash Column Chromatography is pressure- and not vacuum driven.Īlthough we have never experienced any problems, it is important to use precaution with evacuated glassware. Liquid chromatography - purification - large scale chromatography
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. Archives
December 2022
Categories |